HORMONES 2015, 14(1):118-125
DOI: 10.14310/horm.2002.1512
Research Paper
Effect of vildagliptin on hsCRP and arterial stiffness in patients with type 2 diabetes mellitus
Ioanna Zografou,1 Christos Sampanis,1 Eugenia Gkaliagkousi,1 Fotios Iliadis,2 Athanasios Papageorgiou,1 Panagiotis Doukelis,3 Konstantinos Vogiatzis,1 Stella Douma1

12nd Propedeutic Department of Internal Medicine, Hippokration Hospital of Thessaloniki; 21st Propedeutic Department of Internal Medicine, AHEPA Hospital of Thessaloniki, Aristotle University of Thessaloniki; Greece; 3Department of Internal Medicine, Western General Hospital, Edinburgh, UK

Abstract

OBJECTIVE: To evaluate the effect of dipeptidyl-peptidase-4 (DPP-4) inhibitor vildagliptin on high sensitivity C-reactive protein (hsCRP) and arterial stiffness (AS) in patients with type 2 diabetes (T2DM).
DESIGN: Sixty-four drug-naive diabetic patients, with inadequate glycemic control, participated in this randomized, open-label study. Half of the patients received metformin 1700 mg/d and the other half of them received metformin 1700 mg/d plus vildagliptin 100 mg/d. AS was measured by carotid-femoral Pulse Wave Velocity (cfPWV). Body weight (BW), body mass index (BMI), blood pressure (BP), hsCRP, glycosylated hemoglobin (HbA1c), fasting plasma glucose (FPG), lipid profile, albumin/creatinine ratio (ACR), fasting insulin, C-peptide, homeostasis model assessment of insulin resistance (HOMA-IR) and homeostasis model assessment of β-cell function (HOMA-β) were also assessed at baseline and after 6 months.
RESULTS: Vildagliptin in combination with metformin had a beneficial influence on hsCRP, HbA1c, C-peptide and HOMA-β index (p <0.05) but had no effect on cfPWV, BP, BW, BMI, lipid profile, ACR and HOMA-IR compared with metformin alone (p=NS).
CONCLUSIONS: We have found that the addition of vildagliptin to metformin for a period of six months decreased hsCRP, improved glycemic control and β-cell function but had no effect on AS in drug-naive patients with T2DM.

Keywords

Arterial stiffness, Dipeptidyl-peptidase-4 inhibitor, High sensitivity C-reactive protein, Pulse wave velocity, Vildagliptin


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INTRODUCTION

Diabetes mellitus is a chronic disease associated with a two- to fourfold increased risk of cardiovascular disease (CVD).1,2 Although antihyperglycemic drugs improve glycemic control the UKPDS (United Kingdom Prospective Diabetes Study) has found that metformin may confer cardiovascular benefits to certain subgroups of patients while the PROactive study (PROspective pioglitazone Clinical Trial In macroVascular Events) found similar beneficial effects of pioglitazone.3,4 However, there is still not sufficient evidence that antidiabetic therapies reduce the cardiovascular risk of patients with diabetes.

Incretin-based therapy has been approved for the treatment of type 2 diabetes mellitus (T2DM). Incretins are a group of gastrointestinal hormones, among which the glucagon-like peptide 1 (GLP-1) and the glucose-dependent insulinotropic peptide (GIP), which are released in response to food ingestion, stimulating insulin secretion. This action, however, is limited by their rapid inactivation by the enzyme dipeptidyl peptidase-4 (DPP-4).5,6 Moreover, incretin hormones suppress glucagon secretion and seem to have some beneficial effect on β-cells function in experimental models.7 DPP-4 inhibitors reduce the breakdown of GLP-1 and improve glycemic control by increasing insulin secretion.8

Beyond its favorable action on metabolic control, GLP-1 seems to exert beneficial cardiovascular effects, while it also has vasodilatory properties thought to be partially mediated through a specific GLP-1 receptor on the vascular endothelium. Administration of inherent GLP-1 caused dilation of the aorta wall in animals.9 Probably some of the effects of GLP-1 are mediated through its metabolites and are independent of the GLP-1 receptor, acting instead through an NO/cGMP-dependent mechanism.10

Vildagliptin is an oral agent that selectively inhibits DPP-4, increases levels of active GLP-1 and GIP and improves glycemic control in patients with T2DM.11 Moreover, vildagliptin decreases adipocytokines related to inflammation12 and inhibits monocyte activation/chemotaxis.13 Recent studies have investigated the anti-inflammatory and antiatherosclerotic properties of DPP-4 inhibition and also the improvement in endothelium-dependent vasodilatation in subjects with T2DM, suggesting that this class of drugs might have favourable cardiovascular implications.14

Arterial stiffness (AS) is a strong predictor of cardiovascular morbidity and all-cause mortality in different populations and it is an independent predictor of 10-year mortality in patients with diabetes.15-17 High sensitivity CRP (hsCRP) as a marker of inflammation is reported to be independently related to an increased AS.18 The relationship between the presence of systemic inflammation and increased aortic stiffness has been documented in many studies involving both acute and chronic inflammation.19,20

Based on previous observations, we hypothesized that treatment with vildagliptin might beneficially affect inflammation and AS in patients with T2DM. We measured carotid-femoral pulse wave velocity (cfPWV), which is considered to be the most appropriate and accurate non-invasive method to quantify AS. Furthermore, this method is simple and reproducible and has been demonstrated to be a reliable predictor of cardiovascular mortality in different populations.21,22 Although vascular function has been studied in patients with diabetes, data concerning the effect of vildagliptin on hsCRP and AS are still lacking. The aim of this study was to investigate if 6 months treatment with vildagliptin would affect hsCRP and AS in drug-naive patients with T2DM.

PARTICIPANTS AND METHODS

Sixty-four drug-naive diabetic patients, aged 54.4±10.9 years (mean±SD) (38 men, 26 women), were recruited from our outpatient diabetic clinic in this prospective, open-label, randomized study. All participants were Caucasians. Inclusion criteria were 1) patients ≥18 and ≤70 years old and 2) inadequate glycemic control [glycosylated hemoglobin (HbA1c) 7-9%] despite diet and exercise for 3 months. Patients with any macrovascular or microvascular diabetic complications, history of heart disease, uncontrolled hypertension [systolic Blood Pressure (BP) ≥180 mmHg and/or diastolic BP ≥110 mmHg], excessive dyslipidemia [total cholesterol (TC) ≥350 mg/dl, triglycerides (TG) ≥400 mg/d], elevated liver enzymes three times above the upper normal range and any other comorbidity that could interfere with the study were excluded. Pregnant women or those who were breastfeeding or planning for pregnancy were also not eligible. Women of childbearing age were advised to use contraception if they were sexually active.

The study was approved by the local Ethics Committee (Ethics Committee of Aristotle University of Thessaloniki) and conducted in accordance with the Helsinki Declaration. Informed consent was obtained from every participant.

Enrolled patients were assigned randomly either to receive metformin (850 mg twice daily) or to receive metformin (850 mg twice daily) plus vildagliptin (50 mg twice daily) for 6 months. Also, the antihypertensive and lipid-lowering drugs were unchanged during the study period. Patients in both groups were instructed to strictly maintain dietary habits and daily activities during the course of the study.

Participants were assessed at the outpatient clinic two times: at baseline and at 6 months. Blood samples for biochemical measurements were collected and cfPWV was measured at baseline and at the end of the study.

Adverse events were monitored throughout the study. Hypoglycemia was defined as capillary blood result <70mg/dl and/or signs or symptoms of hypoglycemia resolving after carbohydrate intake.

CfPWV was measured by the SphygmoCor system by sequential recording electrocardiogram-gated carotid femoral waveforms. The same investigator performed all measurements. The patients were informed not to take any medicine on the morning of their visit and smokers also not to smoke. BP was measured at the brachial artery using a semiautomatic device (Omron digital tonometer) after 10 min rest in the seated position. CfPWV was performed in a quiet, well ventilated room after 10 min resting in the supine position. The distance from the lowest point of the sternal notch to the carotid and femoral artery sites was obtained. Waveforms were captured from the carotid and femoral arteries using the tonometer connected to the SphygmoCor device. Waveforms were gated by the R wave on the simultaneously recorded electrocardiogram and PWV was measured after at least 15 sequential waveforms. The average of three recordings of cfPWV was used in the analysis.

Body weight (BW) was measured with light clothing on a balance scale and height to the nearest 0.5 cm using a meter that was stabilized on the wall. BMI was calculated as weight (kilograms) divided by height (meters) squared. Percentage fat mass was measured by body fat analyzer (Tanita TBF-531 body fat scale). Waist circumference was measured at minimal respiration by a flexible tape parallel to the floor and immediately above the iliac crest.

All laboratory tests were performed after a 12 h overnight fast. Biochemistry, including glucose, lipid levels (TC; high density lipoprotein cholesterol, HDL-C; and TG) liver enzymes (alanine transaminase, ALT; aspartate transaminase, AST) and creatinine, were evaluated on an Olympus analyzer (Olympus Diagnostica GmbH, Clare, Ireland). Low-density lipoprotein cholesterol (LDL-C) was calculated using Friedewald’s formula.23 HbA1c and albumin/creatinine ratio (ACR) were measured by an automated latex immunoagglutination inhibition assay (DCA 2000, Bayer Diagnostics Europe Ltd., Dublin, Ireland). Estimated glomerular filtration rate (eGFR) was calculated according to the Gault-Cockroft equation (eGFR (ml/min/1.73 m2) = [(140-Age) × BW (in kg)] [72 × Serum creatinine (in mg/dL)] × (0.85 if female).

HsCRP was quantitated by an immunoassay (Quantikine Human C - reactive Protein Immunoassay, R&D Systems Europe Ltd, UK) (normal range, 0.1-4.3 mg/L). Inter-assay and intra-assay coefficients of variation for hs-CRP averaged 6% and 4.4%, respectively. Insulin was quantitated by an immunoradiometric assay (INS-IRMA, DIAsourceImmunoAssays S.A., Louvain-la-Neuve, Belgium) (normal range, 2.6–24.9 μU/ml). Inter-assay and intra-assay coefficients of variation for insulin averaged 6.5% and 2.1%, respectively. C-peptide was quantitated by a radioimmunoassay (C-PEP RIA-CT, DIAsourceImmunoAssays S.A., Louvain-la-Neuve, Belgium) (normal range 0.51-2.72 ng/ml). Inter-assay and intra-assay coefficients of variation averaged 11.8% and 5.6%, respectively. Insulin resistance was estimated by homeostasis model assessment of insulin resistance, HOMA-IR [FBG (mmol/L) x fasting insulin (μU/ml)/22.5] and β-cell function was assessed by homeostasis model assessment of β-cell function, HOMA-β [20 × fasting insulin (μU/ml)/FBG (mmol/L) - 3.5].24

STATISTICAL ANALYSIS

All data were analyzed using the statistical package SPSS (version 17; SPSS Inc., Chicago, Illinois, USA). Normality of distribution was assessed by the Kolmogorov-Smirnov test. Continuous data are presented as mean ± 1 SD and categorical data as absolute numbers and percentages. Comparisons within groups were performed by the paired t test and Wilcoxon Signed rank test and between groups by the independent t test and Mann-Whitney U test for normal and non-normal distributions, respectively. The Chi-square test was used for comparisons of categorical variables between groups. Pearson’s and Spearman’s correlation coefficients were used to detect dependence between normally and non-normally distributed variables, respectively. In all cases, a 2-tailed p value less than 0.05 was considered statistically significant.

RESULTS

Sixty-four patients (38 men and 26 women) participated in the study. Baseline characteristics are presented in Table 1. No significant differences were observed between groups at baseline.

All results are summarized in Τable 2. The combination of vildagliptin with metformin was associated with a significant decrease in hsCRP compared with metformin alone (vildagliptin plus metformin group at baseline 3.4±2.8 mg/L, Δ at 6 months -0.4±2.8 mg/L, p<0.05, metformin group 2.5±2.3 mg/L, Δ at 6 months 1.1±1.6 mg/L, p<0.05 between groups). In addition, HbA1c was decreased more effectively in the same group (p<0.05 for difference within groups and between groups) and HOMA-β and C-peptide were also beneficially affected (p<0.05 for difference within vildagliptin plus metformin group and between groups). There was no correlation between the hsCRP reduction and the HbA1c, HOMA-β and C-peptide improvement in the vildagliptin plus metformin group (p=NS). No effect was found on cfPWV (no statistical difference within groups and between groups: cfPWV vildagliptin plus metformin group at baseline 8.6±2.1 m/sec, Δ at 6 months -0.3±1.5 m/sec, p=NS, metformin group 8.9±2 m/sec, Δ at 6 months 0.2±1.8 m/sec, p=NS). BW, BMI, HOMA-IR and fat mass percentage were reduced in both groups (p<0.05 for difference within groups), but without difference between groups. There was no statistically significant difference in lipid profile, BP, eGFR and fasting insulin within and between groups. Although vildagliptin reduced ACR (p<0.05 for difference within group), the difference between groups was not significant. There was a significant reduction in liver enzymes in the metformin group but with no difference between groups at the end of study.

Table 2

No serious adverse event was reported. One case of urinary tract infection and one case of upper respiratory tract infection was observed in the metformin group. In the vildagliptin plus metformin group there was one case of nasopharyngitis.

DISCUSSION

In our study, the addition of vildagliptin to metformin decreased hsCRP independently of glycemic control but it had no effect on AS in drug-naive patients with T2DM after 6 months.

Cardiovascular disease is the leading cause of death in patients with diabetes due to premature atherosclerosis and arterial complications. Inflammatory mediators, such as hsCRP or IL-6 are increased in diabetes18 and inflammation seems to play a key role in the pathogenesis of vascular dysfunction.25 In addition, hyperglycemia promotes the formation of advanced glycation end-products and the glycation of proteins resulting in changes in elastin and inelastic matrix materials located in the vessel wall and finally their stiffening.26

Vascular function has been investigated in patients with T2DM and it has been shown that these patients have increased AS, reduced aortic distensibility and impaired endothelial function, as this is estimated by the method of flow-mediated dilation, when compared to nondiabetic population matched for sex, age and serum total cholesterol.27,28

The assessment of inflammatory burden and vascular function in the early stage of diabetes might be of great prognostic importance. Previous studies have shown that AS is a strong predictor of cardiovascular morbidity and all-cause mortality in different populations and that it is an independent predictor of 10-year mortality in patients with diabetes.13,15,16 Among the different methods to assess AS, cfPWV is the most robust one. This method is useful not only for prediction of future cardiovascular events but also for risk stratification and probably for early therapeutic intervention in patients with diabetes.29

Besides glycemic control, new treatment strategies for diabetes have focused on the reduction of cardiovascular disease and its complications. There is a growing body of evidence coming from experimental and small clinical trials that GLP-1-mimetics therapies have antiatherosclerotic and antiremodeling properties beyond glycemic control.30,31 Apart from GLP-1, its degradation product, GLP-1 (9-37), was found to have beneficial effects on the cardiovascular system and vascular tone as well.32 It remains to be seen if GLP-1 increase by DPP-4 inhibition exhibits the same vascular profile.

Although two recently published studies have shown that vildagliptin improved endothelium-dependent vasodilatation in subjects with T2DM and blocked vascular injury of the thoracic aorta in diabetic rats,13,33 there is no sufficient data regarding the effect of vildagliptin on hsCRP and AS in patients with diabetes until now.34,35 We hypothesized that vildagliptin might reduce systemic inflammation and, as a consequence, improve AS. Previous studies have provided convincing evidence that there is a relationship between hsCRP levels and AS.36,37 However, this relationship was not confirmed in our study, as vildagliptin failed to improve cfPWV during the 6-month follow-up. This might be due to the short (6 months) duration of therapy which was inadequate to induce any beneficial structural changes in arterial walls. Furthermore, although vildagliptin improved the glycemic profile of the patients, it had no effect on parameters such as BP and BMI which greatly influence PWV. The lack of any amelioration in these factors may explain the absence of functional changes in the arterial walls that would result in improvement of arterial distensibility and thereby reduce PWV. It is also possible that a larger cohort of patients is needed in order to establish an independent correlation among hsCRP levels and AS.

Metformin was found to reduce cfPWV after 3 months in young women with polycystic ovary syndrome. However, these results are not comparable because these women did not have T2DM, were younger (mean age 31) and had lower baseline PWV.38 On the other hand, in another metformin study, in a non-diabetic African - American population with insulin resistance and treated with metformin for 24 months, a significant increase in PWV was observed when compared with placebo.39

Vildagliptin increased insulin secretion and improved β-cell function (improvement in C-peptide and HOMA-β index, p <0.05). The same effect of vildagliptin on glycemic control has been demonstrated in another study.40 There was no change in fasting insulin, HOMA-IR, lipid profile, BP and ACR. Several studies have evaluated the effects of GLP-1 based therapies on preservation of β-cell function. There is some evidence that vildagliptin treatment ameliorates β-cell dysfunction in patients with T2DM as well as impaired fasting glucose. This could offer an alternative approach in preventing the progression of diabetes.41,42

The addition of vildagliptin to metformin had no effect on BW, BMI, fat mass and FPG. The neutral effect of vildagliptin on BW and BMI is in agreement with previous studies and it can be explained by the fact that unlike mimetics, which boost GLP-1 activity this leading to BW changes, DPP-4 inhibition works by slowing incretins degradation, therefore the level of circulating GLP-1 in inhibition therapy is significantly lower than that of mimetics therapy.43

It should be noted here that there are several limitations in our study. The most important limitation is the small number of subjects. Also, it was open-label and perhaps the period of intervention was too short to affect AS. Thus, our results should be interpreted with caution and, undoubtedly, long-term placebo-controlled studies are needed.

In conclusion, we have found that the addition of vildagliptin to metformin given for a period of 6 months decreased hsCRP and improved glycemic control and β-cell function but had no effect on AS in drug-naive patients with T2DM. Further large randomized placebo controlled long-term studies are required to evaluate the effect of vildagliptin on inflammation and vascular function in patients with T2DM.

FINANCIAL DISCLOSURES

None declared.

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Address for correspondence:
Ioanna Zografou, MD, Second Propedeutic Department of Internal Medicine, Hippokration Hospital, 49 Konstantinoupoleos Str., 54642, Thessaloniki, Greece, Tel.: +302310892631, E-mail: Ioannazo@yahoo.gr

Received 21-01-2014, Accepted 19-03-2014